Journal: Nature Communications
Article Title: High-quality metagenome assembly from nanopore reads with nanoMDBG
doi: 10.1038/s41467-026-69760-y
Figure Lengend Snippet: The top row presents results for Nanopore data, and the bottom row for HiFi data. a Contamination level in contigs longer than 1 Mbp. The percentage above each plot indicates the overall proportion of long contaminated contigs (with contamination over 5%). b Number of clipping events supported by at least 10 reads. c Number of regions over 1,000 bp with no apparent read coverage. d Proportion of predicted proteins which are ≥90% the length of their best-matching known protein in a reference database (from contigs with more than 10x coverage). In ( a ), results are aggregated across assemblies of the three data sets: Human Gut, Zymo Fecal Reference and Soil, but separated by both data set and assembler in b , c , and d . The boxplot elements are the median (horizontal bar), 25th and 75th percentiles (box limits Q1 and Q3), Q1-1.5*IQR and Q3+1.5*IQR (whiskers, IQR = Q3 − Q1) and outliers. Summary statistics in ( a ) (n, min, max, median, 25th and 75th percentiles, lower whisker, upper whisker): Nanopore-nanoMDBG (1006, 0, 28.47, 0.15, 0.04, 0.46, 0, 1.09); metaMDBG (549, 0, 28.07, 0.18, 0.05, 0.6, 0, 1.37); metaFlye (326, 0, 18.56, 0.13, 0.03, 0.39, 0, 0.92): HiFi-metaMDBG (1239, 0, 28.27, 0.14, 0.04, 0.41, 0, 0.96); hifiasm-meta (667, 0, 23.3, 0.15, 0.05, 0.445, 0, 1.03); metaFlye (362, 0, 20.41, 0.14, 0.04, 0.4, 0, 0.93).
Article Snippet: Zymo mock reference genomes are available at https://s3.amazonaws.com/zymo-files/BioPool/D6331.refseq.zip .
Techniques: Whisker Assay